Effect of VA on the expression levels of p53, p21, cyclin D1 and cyclin E in MCF-7 and MDA-MB-231 cells.

<p>Time-dependent regulation by VA in (A) MCF-7 cells and (C) MDA-MB-231 cells. Cells were treated with VA (50 µg/ml) for 48 h. The data are representative of at least three independent experiments. (B) and (D) Protein quantification of the western blot results shown in (A) and (C) respectively. Protein levels were normalized to the β-actin level and are shown relative to the DMSO-treated control cells (normalized at 1). Statistical differences were analyzed with one-way ANOVA test. **<i>p</i><0.01, *** <i>p</i><0.001.