Increased Nox4 protein expression in cultured aortic endothelial and smooth muscle cells of Bmal1-KO mice.

<p>Vascular smooth muscle and endothelial cells were isolated and cultured from aortae of WT and Bmal1-KO mice (passage 2-3). Nox4 expression levels were determined by immunoblotting and revealed a significant increase in Nox4 in vascular endothelial cells (<b>A</b>) and smooth muscle cells (<b>B</b>) from Bmal1-KO animals relative to wild-type mice. Changes were quantified by densitometry (^*p<0.05 versus WT, n=3).