Administration of uric acid to mice results in an abnormal response to glucose.

<p>(<b>A</b>) Serum uric acid levels of mice were significantly increased after daily injection of uric acid (250 mg/kg) for 4 weeks. (<b>B</b>) This figure showed the results of glucose tolerance test. The glucose tolerance test (2 mg/kg) led to significantly elevated blood glucose levels in hyperuricemic mice compared to controls. (<b>C</b>) This figure showed the results of insulin tolerance test. In the insulin tolerance test (0.75 U/kg), there was no difference in blood glucose levels between hyperuricemic mice and control mice. (<b>D</b>) Plasma insulin level was significantly lower in hyperuricemic mice at 30 min than in the control group. (<b>E</b>) Immunohistology using antibody to insulin showed reduced insulin content in islets from hyperuricemic mice compared with that of control mice (original magnification,×200). (<b>F</b>) Fold changes in insulin content were determined in hyperuricemic mice and control mice. Insulin content was significantly reduced in hyperuricemic mice. (<b>G</b>) Fold changes in islet area were determined in hyperuricemic mice and control mice. Islet area was significantly reduced in hyperuricemic mice. *<i>P</i><0.05, **<i>P</i><0.01.