DcR3 reduced gemcitabine-induced apoptosis.

<p>Gemcitabine was added to media of AsPC-1 (500 nM) or MiaPaCa-2 cells (50 nM) incubated with 0, 0.5, 1, or 2 μg/ml of recombinant DcR3 for 24 hours. Cells were then subjected to flow cytometry for the sub-G1 fraction (<b>A</b> and <b>B</b>) or an enzyme assay for caspase 3 activity (<b>C</b> and <b>D</b>). Gemcitabine-triggered apoptosis was decreased by the addition of DcR3 in a dose-dependent manner (<i>P</i><0.01).