Quantitative RT-PCR (qRT-PCR) and Western blot analysis of several putative miRNAs target genes related to steroidogenesis.

<p>(A) qRT-PCR analyses of selected genes in rat adrenal glands regulated by ACTH, 17α-E2 and DEX. (B) qRT-PCR analyses of Bt₂cAMP regulated genes in rat granulosa cells. 36B4 was used for normalization. *p<0.05; **p<0.01; ***p<0.001. C. Western blot analysis of SREBP-1c, SF-1, HDAC3, StAR, LDLR and β-actin in different treated rat adrenals and rat granulosa cells.