Effect of AE-BCT on NF-κB activation in HT1080 cells.

<p>(<b>A</b>) Control and AE-BCT-pre-treated HT1080 cells were stimulated with 5 nM PMA for the indicated periods of time and cell lysates were subjected to Western blotting of the phosphorylation and degradation of IκBα. (<b>B</b>) To examine the nuclear translocation of the NF-κB p65 subunit in response to PMA stimulation, cell lysates were fractionated into cytosolic and nuclear compartments, and subjected to Western blotting. After normalization to α-tubulin or TBP expression, relative ratios of pIκBα/IκBα and nuclear p65/cytosolic p65 were determined. Data are expressed as means ± SD of two independent experiments. **<i>p</i><0.01 <i>vs</i> no AE-BCT.