<p>(A) and (B) J774A.1 macrophages (2×10<sup>6</sup> in 2 ml of medium) were incubated for 30 min with or without the indicated concentration of osthole and for 6 h with or without addition of 1 µg/ml of LPS, then expression of NLRP3 (A) and pro-IL-1β (B) was analyzed by Western blotting. (C) and (D) J774A.1 macrophages (2×10<sup>6</sup> in 2 ml of medium) were incubated for 30 min with or without the indicated concentration of osthole, then for 6 h with or without addition of 1 µg/ml of LPS. After washing, the cells were incubated with or without 5 mM ATP for 30 min, then IL-1β in the culture medium was measured by ELISA (C) and active caspase-1 (p10) and caspase-1 (p45) in the cells measured by Western blotting (D). (E) and (F) J774A.1 ma...
Inflammation underlies the pathology of numerous diseases. It can be initiated by macrophages throug...
<p>A, Levels of ROS in <i>M.hy</i> treated THP-1 cells were analyzed by CM-H2DCFDA labeling. Data re...
<p>(A) Mesangial cells (3×10<sup>4</sup> in 1 ml of medium) were incubated for 2 h with or without o...
<p>(<b>A</b>) J774A.1 macrophages (1 × 10<sup>6</sup>/ml; 1 ml) or (<b>B</b>) peritoneal macrophages...
<p>(<b>A</b> and <b>B</b>) J774A.1 macrophages were incubated for 30 min with or without 2 μM of GW-...
<p>LPS primed microglia were stimulated with ATP (1 mM, 30 min), Nigericin (Nig, 1.34 μM, 2 h), Mono...
<p>(A–C) After priming with or without LPS (100 ng/mL) for 6 h, J774 macrophages were treated with o...
<p>A, 1×10<sup>5</sup> PMA-induced macrophages were primed with 50 ng/ml LPS, then treated with <i>M...
NLRP3 inflammasome activation provides an innate host defence against pathogens. Two steps of inflam...
Inflammation is a key factor in the pathogenesis of ALI. Therefore, suppression of inflammatory resp...
Real-time PCR analysis of the NLRP3 inflammasome components, NLRP3 (A), ASC (B), and caspase-1 (C) i...
<p>A. Cells were cultured with PBS (control) or with ox-LDL (100 µg/ml, 48 h). Gene expression of in...
IL-1β release from WT and NLRP3-deficient BMDM (A) or PMs (B) primed with LPS (200 ng/ml, 3 h), then...
Inflammation is an important response of the innate immune system that aids in recruiting immune cel...
<p>(A) Mature NLRP3 foci in infected THP-1 cells. Differentiated THP-1 cells were uninfected (UI) or...
Inflammation underlies the pathology of numerous diseases. It can be initiated by macrophages throug...
<p>A, Levels of ROS in <i>M.hy</i> treated THP-1 cells were analyzed by CM-H2DCFDA labeling. Data re...
<p>(A) Mesangial cells (3×10<sup>4</sup> in 1 ml of medium) were incubated for 2 h with or without o...
<p>(<b>A</b>) J774A.1 macrophages (1 × 10<sup>6</sup>/ml; 1 ml) or (<b>B</b>) peritoneal macrophages...
<p>(<b>A</b> and <b>B</b>) J774A.1 macrophages were incubated for 30 min with or without 2 μM of GW-...
<p>LPS primed microglia were stimulated with ATP (1 mM, 30 min), Nigericin (Nig, 1.34 μM, 2 h), Mono...
<p>(A–C) After priming with or without LPS (100 ng/mL) for 6 h, J774 macrophages were treated with o...
<p>A, 1×10<sup>5</sup> PMA-induced macrophages were primed with 50 ng/ml LPS, then treated with <i>M...
NLRP3 inflammasome activation provides an innate host defence against pathogens. Two steps of inflam...
Inflammation is a key factor in the pathogenesis of ALI. Therefore, suppression of inflammatory resp...
Real-time PCR analysis of the NLRP3 inflammasome components, NLRP3 (A), ASC (B), and caspase-1 (C) i...
<p>A. Cells were cultured with PBS (control) or with ox-LDL (100 µg/ml, 48 h). Gene expression of in...
IL-1β release from WT and NLRP3-deficient BMDM (A) or PMs (B) primed with LPS (200 ng/ml, 3 h), then...
Inflammation is an important response of the innate immune system that aids in recruiting immune cel...
<p>(A) Mature NLRP3 foci in infected THP-1 cells. Differentiated THP-1 cells were uninfected (UI) or...
Inflammation underlies the pathology of numerous diseases. It can be initiated by macrophages throug...
<p>A, Levels of ROS in <i>M.hy</i> treated THP-1 cells were analyzed by CM-H2DCFDA labeling. Data re...
<p>(A) Mesangial cells (3×10<sup>4</sup> in 1 ml of medium) were incubated for 2 h with or without o...