Effect of corticosteroid receptor gene knockdown on HRC number.

<p>Zebrafish embryos at the 1∼4 cell-stage were microinjected with glucocorticoid receptor ATG-MO (GR-ATG), GR-splice variant MO (GR-SV), mineralocorticoid receptor ATG-MO (MR-ATG), or Random-MO (RMO; control). Representative images of yolk-sac HRCs labeled with H⁺-ATPase (HA) in RMO (<i>A</i>), GR-ATG (<i>B</i>), GR-SV (<i>C</i>), and MR-ATG (<i>D</i>) morphants. HRC numbers are compared in (<i>E</i>). Values are presented as the mean ±s.d. (n = 10–12). ^abIndicates statistically significant differences (<0.05) between groups as determined by one-way ANOVA (Tukey’s pair-wise comparison). Scale bar: 100 µm (<i>A–F</i>).