Distribution of newborn cells in the adult hypothalamus.

<p>BrdU incorporation into hypothalamic cells as a measure of cell proliferation was assessed after a single icv injection of BrdU (<b>A</b>), a 7 day icv infusion (<b>B</b>), or a 28 day exposure to BrdU-containing drinking water (<b>C</b>). Arrows in <b>A</b> indicate BrdU+ doublets. (<b>D</b>), distribution of hypothalamic BrdU+ cells at day 32 after the initiation of a 7 day icv BrdU infusion. (<b>E</b>), labelling for both BrdU (red) and NG2 (green) reveals numerous BrdU positive NG2-glia cells. Boxed area is shown enlarged to the right, arrows mark BrdU+ cells. Scale bars = 200 µm.