eGFP expression from a monocopy randomly inserted P_inR-<i>egfp</i> fusion in planctonic cells of <i>P. knackmussii</i> B13 grown in batch culture and sampled in exponential phase (A) or stationary phase (B).

<p>Micrographs show typical population differences of cells grown on 5-chlorobenzoate (3CBA) under non-inducing (exponential phase) and inducing conditions (stationary phase), taken under eGFP illumination (right) and the corresponding image in phase contrast (PhC, left). The white bar in images corresponds to a scale of 10 µm. Graphs show fluorescence values (AGVs) measured from single cells represented as histograms and lateral boxplots (grey area below graph). Percentages correspond to calculated sizes of subpopulations statistically significantly expressing eGFP. Note that the calculated mean fluorescence values over the whole population are statistically significantly different if assuming both are normally distributed (P = 0.00056, Welch two-sample t-test).