G1 arrest is initiated before the diauxic shift.

<p>(A) Culture density based on optical density at 600 nm wavelength (OD600), (B) cell number increase, (C) percentage of cells in G1 are plotted for wild type cells grown in YEPD medium from log to stationary phase (stationary phase.) Values are the average of four growth curves and error bars are included. Cell number after the DS (14 hr) and at the end of cell division are indicated. (D) DNA fluorescence shown as scatter plots and histograms of log phase wild type cells, cells at the DS, and one hour later.