HLA-DRα is down-regulated in LANA-expressing B lymphoma cells.

<p>(<b>A</b>) The mRNA level of HLA-DRα was reduced in LANA-expressing BJAB cells. The total RNA of BJAB cells with LANA-RFP or vector RFP stable expression were subjected to microarray assay. Representative data and average ratio of HLA-DRα, CIITA and CREB from LANA-RFP and RFP microarray assay are shown. (<b>B</b>) Northern analysis of HLA-DRα transcript. The total RNA of BJAB and DG75 cells individually transduced with lentiviruses carrying LANA (YLF) or vector (GFP) alone was subjected to northern blot as described in material and method. The relative density of HLA-DRα transcript is verified by quantitative PCR. The efficiency of lentivirus transduction is shown at the right panels. (<b>C</b>) LANA inhibits the expression of HLA-DRα. Ten million of DG75 cells were electroporated with pA3M-LANA (5, 10, 15 µg) or Vector pA3M. At 48-hr post-transfection, the cell lysate were separated by SDS-PAGE and analyzed by Western blot using antibodies HLA-DRα or myc(9E10). Detection of GAPDH was used as an internal control. (<b>D</b>) The surface expression of HLA-DR proteins on DG75 cells transfected with LANA-myc or vector was determined by FACS using PE-labeled mAb against HLA-DR.