CAP257 broadly neutralizing antibodies develop sequentially in three distinct waves.

<p><b>A</b>) Longitudinal neutralization of the autologous CAP257 virus (black) and 37 heterologous viruses neutralized by CAP257 plasma at titers >1∶100. The ID₅₀ titers (y-axis) are shown versus weeks p.i. (x-axis). Three peaks in heterologous neutralization titers at 67, 122, and 213 weeks p.i. are indicated with dotted lines. Heterologous viruses are colored according to subtype (A = green, B = blue, C = red). <b>B</b>) A summary of the three waves of heterologous neutralization defined by a representative virus, superimposed over the neutralization kinetics shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003738#ppat-1003738-g001" target="_blank">Figure 1A</a>. Wave 1 was subtype C specific and is colored red. Wave 2 neutralized viruses from all three clades and is colored green. Wave 3 is colored brown. <b>C</b>) Adsorption of heterologous neutralization at the peak of each of the three waves. Percentage inhibition (y-axis) is shown versus plasma dilution (x-axis). Untreated plasma is shown in black, blank beads in grey and beads coated with recombinant proteins are shown in red (wave 1), blue/green (wave 2) or brown (wave 3).