DENV2 E85-VRP-immunization reduced viral load during antibody-mediated severe dengue disease.

<p>AG129 mice were immunized with 1×10⁶ IU DENV2 E85-VRP i.p. 14 and 5 days prior to challenge with 5×10⁸ GE DENV. The baseline and ADE groups were included as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003723#ppat-1003723-g001" target="_blank">figure 1</a>. Viral RNA levels were measured in the liver 3 days after challenge. (A) One group of immunized mice was challenged in the presence of exogenous anti-DENV antibody 2H2 (black diamonds), and another group of immunized mice was challenged in the presence of a non-specific isotype control C1.18 (black triangles). (B) One group of mice was immunized with 1×10⁶ IU VRP expressing GFP (VRP-GFP, white triangles) instead of the DENV-E ectodomain (DENV2 E85-VRP, black triangles). Each symbol depicts one mouse, P-values from two-tailed unpaired t-test with Welch's correction, confidence interval 95%, * P≤0.05, ** P≤0.01, *** P≤0.001. Dotted line and symbols in gray as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003723#ppat-1003723-g001" target="_blank">figure 1</a>.