Serglycin is highly expressed in aggressive breast cancer cell lines.

<p>(A) Expression of serglycin across the 51 individual breast cancer cell lines grouped in the basal A (red), basal B (grey) and luminal (blue) subgroups. (B) Detection of serglycin gene transcripts in low aggressive MCF-7 (luminal), medium aggressive MDA-MB-468 (basal A) and high aggressive MDA-MB-231 (basal B) breast cancer cells (bold and underlined in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0078157#pone-0078157-g002" target="_blank">figure 2A</a>) cultured in the presence or absence of serum by RT-PCR. The PCR products were analyzed on 1% agarose gels stained with GelRed. (C). Subcellular distribution of serglycin in breast cancer cells. Immunofluorescence staining was done for serglycin (green), nuclei (blue) and F-actin (red) in high aggressive MDA-MB-231 and low aggressive MCF-7 breast cancer cells after permeabilization. MCF-7 cells stained faintly for serglycin in the cytoplasm. Serglycin was found in the perinuclear regions and juxtanuclear area typical of the Golgi complex in MDA-MB-231 cells (arrowheads), and in the cytoplasm and at the cell periphery (arrows). Serglycin distribution was observed in vesicular (V) and vesiculotubular structures (VST) in the cytoplasm (insert). Bars, 25 µm.