Mechanism of arginase activity induction by <i>T. brucei</i> PRF and rTbKHC1.

<p>Myeloid cells from non-infected WT (<b>A-D,F, G</b>) or IL-4Rα KO (<b>E</b>) mice were incubated with PRF or rTbKHC1. (<b>A</b>) IL-10 production induced by PRF. (<b>B</b>) Arginase activity induced by PRF incubated with indicated antibodies or sugars. (<b>C</b>) Arginase activity and IL-10 secretion induced by rTbKHC1 incubated with indicated antibodies or sugars. (<b>D–G</b>) Relative expression level of M2 genes after incubation with rTbKHC1. In <b>F</b>, M2 IL-10-dependent genes are indicated, and in <b>G</b>, rTbKHC1 was incubated with the indicated antibodies. Data are means ± SEM of 3 individual mice of one representative from 3 independent experiments. * p<0.05 compared to non-stimulated (-) cells.