The binding to zinc ions by ZF1 or/and ZF2 is not required for ZNF24 nuclear localization.

<p>(A) Schematic of different ZNF24-GFP mutant constructs and are denoted by an asterisk. The zinc-chelating histidines of ZF1 or/and ZF2 are mutated to leucine, respectively. The shaded boxes represent the same features described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079910#pone-0079910-g001" target="_blank">Figure 1</a>. (B) Subcellular localization of mutant ZNF24-GFPs. Indirect immunofluoresence was conducted on the indicated constructs and analyzed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079910#pone-0079910-g001" target="_blank">Figure 1</a>. Patterns of localization are summarized on the left. (C) Results (mean±S.E.M., n=100) for quantitative analysis of images to determine the nuclear to cytoplasmic fluorescence ratio (Fn/c).