Procedure for determining the subcellular partitioning of metal.

<p>The liver was homogenized by different treatments and centrifuged at different speeds, afterwards the following subcellular fractionations were obtained: organelles (i.e. nucleus, mitochondria, and microsomes, P2), HSP (heat-sensitive proteins, P3), MTLP (metallothionein-like protein, S3), MRG (metal-rich granules, P4), and cellular debris (S4).