Interference into Revivification of RhoB gene results in inhibition of ovary carcinoma cell apoptosis.

<p>After treated with TSA for 24 hours, SKOV3 and A2780 cells were respectively either nu-transfected (NT), transfected with non-targeted Control siRNA (Control si), or with siRNA duplex against human RhoB (RhoB si). (<b>A</b>) <b>The expression confirmation of RhoB by Western blotting analysis with β-actin used as equal loading control</b>: As shown, transfection with RhoB siRNA, but not with Control siRNA, abrogate the protein expression of RhoB. (<b>B</b>) <b>Apoptosis assays of tumor cells after transfection with RhoB siRNA/Control siRNA following treatment with TSA</b>. TUNEL staining of carcinoma cells revealed that RhoB siRNA, yet not Control siRNA, caused a significant reduction of apoptotic cells in contrast to nu-transfected (NT) carcinoma cells.