Increased nuclear localization of Clb1 promotes FEAR activation during meiosis.

<p>A) Diploid <i>CLB1, CLB1-NES</i> and <i>CLB1-NLS</i> strains bearing <i>spo12Δ</i> and <i>esp1-1</i> or their wild type alleles were allowed to sporulate. Percentage of cells forming dyads, tetrads and monads were calculated after 48 h. B) Cultures of <i>CLB1-ha PDS1-myc₁₈</i>, <i>CLB1-NES-ha PDS1-myc₁₈</i>, and <i>CLB1-NLS-ha PDS1-myc₁₈</i> cells were induced to enter meiosis by transferring them to SPM. Nucleolar separation in cells containing anaphase I and metaphase II spindles was assayed by immunofluorescence. Representative images of cells are shown on the right. C) Data obtained in B are presented graphically. D) Nucleolar separation and nucleolar release of Cdc14 in the sporulating cultures of <i>P_CLB2CDC20</i> (blue), <i>P_CLB2CDC20 P_CLB2CDC55</i> (red), <i>P_CLB2CDC20 P_CLB2CDC55 CLB1-ha</i> (green), <i>P_CLB2CDC20 P_CLB2CDC55 CLB1-NES-ha</i> (purple) and <i>P_CLB2CDC20 P_CLB2CDC55 CLB1-NLS-ha</i> (orange) strains were assayed by immunofluorescence and the data are graphically presented. E) Sample images of cells with nucleolar Cdc14 or Cdc14 released are shown on the right.