FLZ decreased the expression of phospho-APP and APP-CTF.

<p>APP/PS1 double transgenic mice were orally treated with FLZ 150 mg/kg for 20 weeks. SH-SY5Y (APPwt/swe) cells were grown in “stimulating medium” containing 50% DMEM, 50% Opti-MEM, 0.5% FBS, 200 µg/ml G418 and 10 mM butyric acid sodium salt for 12 h to induce the transgene expression. FLZ (0.1, 1 and 10 µM) were incubated with cells for 24 h. Antibodies against p-APP, APP-CTF and APP were subjected to Western blot assay. A representative immunoblot from 4 mice was shown. Results were expressed as mean ±SD. (A) Western blot assay of APP, phospho-APP and APP-CTF in hippocampus of APP/PS1 mice. *<i>P</i><0.05,**<i>P</i><0.01 <i>vs</i>. WT mice; ^#<i>P</i><0.05 <i>vs</i>. APP/PS1 mice. (B) Western blot assay of APP, phospho-APP and APP-CTF in SH-SY5Y (APPwt/swe) cells. **<i>P</i><0.01 <i>vs</i>. Neo SH-SY5Y cells, ^#<i>P</i><0.05, ^##<i>P</i><0.01 <i>vs</i>. solvent-treated SH-SY5Y (APPwt/swe) cells.