Enhanced Nrf2 activity decreases fatty acid transport and results in increased fatty acids content in white adipose tissue.

<p>(<b>A</b>) TG and (<b>B</b>) FFA content were measured in white adipose tissue of fed and fasted C57BL/6 (WT) and Keap1-KD (KD) mice (n=5-6 per group). (<b>C</b>) Nrf2 increased serum glycerol contents in Keap1-KD mice. (<b>D</b>, <b>E</b>, <b>F</b>) The induction of Pparα signaling and fatty acid transporter expression by fasting was attenuated in white adipose tissue of Keap1-KD mice. Total RNA was isolated from fed or fasted C57BL/6 (WT) and Keap1-KD (KD) mice and the relative mRNA levels were quantified by quantitative real-time PCR and normalized with β-2 microglobulin as loading control (n=4-6 per group). (<b>G</b>, <b>H</b>, <b>I</b>) Immunoblot analysis of Pparα and FATP1 in white adipose tissue from fed or fasted C57BL/6 (WT) and Keap1-KD (KD) mice. *, P<0.05, WT-Fasted compared with WT-Fed mice; $, P<0.05, KD-Fed compared with WT-Fed mice; #, P<0.05, KD-Fasted compared with KD-Fed mice; §, P<0.05, KD-Fasted compared with WT-Fasted mice.