<i>In</i><i>vitro</i> growth initiation of non-growing oocytes in PhosSTOP incorporated culture.

<p>(A) <i>In </i><i>vitro</i> growth rates and (B) average diameters of non-growing oocytes 24 hours after culture both were significantly higher in PhosSTOP group compared to those in the control (*<i>P</i><0.05). (C) The non-growing oocytes in the control group showed survival up to day 4 of culture, while in the PhosSTOP group they did not. There was a significant difference between two groups (a^,b<i>P</i><0.05). (D) Microphotograph of non-growing oocytes in the PhosSTOP and control groups after 24 hours of culture. The oocytes in both groups were together transferred in single culture dish for comparing their figures in the photograph. The circles show the oocytes in the PhosSTOP group and the square brace shows ones in the control. Bar=10 µm. (E) Western blotting of phosphorylated Akt shows strongly detection in the specimen from the ovaries cultured with PhosSTOP.