GUS and GFP reporter gene analyses of <i>NDL1</i> expression around the vegetative and reproductive SAMs in <i>Arabidopsis</i> seedlings.

<p>(<b>A</b>) NDL1-GFP localization in three-day-old etiolated seedlings. GFP fluorescence is detectable in the vicinity of the SAM. (<b>B</b>) Panel A with an overlay of the DIC image. (<b>C</b>) and (<b>D</b>) GUS histochemical staining in eight-day-old etiolated seedlings (<b>C</b>) and in ten-day-old seedlings grown in the light (<b>D</b>). GUS staining is not detectable in the SAM. (<b>E</b>) GUS histochemical staining in a longitudinal section of the vegetative SAM in etiolated seedlings. (<b>F</b>) Enlarged view of the SAM from panel E. (<b>G</b>) GUS staining in a mature stamen. (<b>H</b>) Papillar cells showing GUS staining upon pollen germination (the red double-ended arrow points to a germinating pollen tube). Scale bars = 50 µm, Red arrows in panels A, C, D and E indicate cell zones at the periphery of the SAM.