FMNL3 nucleates filopodia formation.

<p>(a) SK-N-AS cells transfected with FH1-FH2 domain FMNL3 clone display abundant filopodia protruding from the surface of the cells. (b) Cells transfected with the FH2 domain only FMNL3 clone do not display filopodia protrusions. TRITC-conjugated phalloidin (red) stains F-actin and DAPI (blue) stains nuclei. (c) SK-N-AS cells transfected with the FH1-FH2 FMNL3 clone displayed significantly enhanced migratory potential compared to cells transfected with the FH2 domain in isolation. Cells displayed no significant alteration in migratory potential when transfected with either the FH1-FH2 or FH2 domain clones in conjunction with either miR-335 or scramble control. (d) Cells transfected with the FH1-FH2 FMNL3 clone also demonstrated increased invasive potential compared to the FH2 domain in isolation. Cells displayed no significant alteration in invasive capacity when transfected with either the FH1-FH2 or FH2 domain clones in conjunction with either miR-335 or scramble control.