Rrp6 overexpression suppresses RNA phenotypes in <i>rrp47∆</i> mutants.

<p>Northern analyses of total cellular RNA isolated from a wild-type strain, an isogenic <i>rrp47∆</i> mutant and from <i>rrp47∆</i> mutants expressing exogenous Rrp6 or Rrp47 from either centromeric (cen) plasmids or 2 micron-based (2μ) constructs. RNA was resolved through 8% denaturing acrylamide gels, transferred to nylon membranes and hybridised with probes complementary to specific RNAs, as follows: (A) U14; (B) snR38; (C) snR13; (D) SCR1; (E) U6; (F) U3; (G) 5.8S; (H) <i>NEL025c</i>; (I) <i>SCR1</i>. Blots shown in A-G and H-I are from distinct gels. Dispersed bands labelled I-pA and II-pA in panels A-C represent snoRNAs that are polyadenylated after termination at sites I and II, respectively. The bands labelled snR13* and U3* are 5’ truncated forms of snR13 and U3. (J) Quantification of signals for the 3’ extended forms of U14, snR38, snR13, U6 and 5.8S, the truncated U3 RNA and the <i>NEL025c</i> mRNA are shown for each strain. Average values of two data sets are normalised to <i>SCR1</i> loading controls and expressed relative to the level of the RNA observed in the wild-type strain.