Sequence comparison between TbWee1 and homolog proteins in other species.

<p>(A) Multiple sequence alignment of the catalytic domains of the putative <i>Trypanosoma brucei</i> Wee1 protein with other Wee1-like kinases. Amino acid sequences were aligned using the ClustalW2 program (<a href="http://www.ebi.ac.uk/clustalw" target="_blank">http://www.ebi.ac.uk/clustalw</a>). Identities are indicated by asterisks below the sequence. Conserved substitutions are marked with two vertical dots and semi-conserved substitutions are marked with a single dot. Dashes represent gaps introduced for optimal alignment. The 11 conserved subdomains are designated by Roman numerals [45,46]. The catalytic and activation segments are indicated with a blue and pink box respectively. A black box indicates the conserved EGD motif. Triangles indicate amino acids that are conserved in all known members of the Wee1 kinase family, but not in other eukaryotic protein kinases. Sequences shown are for Wee1A kinase of <i>Trypanosoma </i><i>brucei</i> (TbWee1, JN083854), humans (HsWee1, NP003381.1), mice (MmWee1, NP033542.2), <i>Schizosaccharomyces </i><i>pombe</i> (SpWee1, NP587933.1), <i>Saccharomyces </i><i>cerevisiae</i> (ScSwe1, NP012348.1), <i>Arabidopsis </i><i>thaliana</i> (AtWee1, NP171796.1), <i>Xenopus laevis</i> (XlWee1, NP001081784.1), and <i>Trypanosoma cruzi</i> (TcWee90, JN573306; TcWee570, JN257712). (B) Comparison of TbWee1 with other protein kinases. The position of the putative protein kinase domain is shown in black and numbers represent the percent amino acid identity with this region of the predicted TbWee1.