Differentiation resistance of <i>Mst-/-</i> ES cells.

<p>(A) Morphology of wild type ES cells and <i>Mst-/</i>- ES cells initially and 24 hour after growing in ES cell differentiation medium supplemented with RA, but not 2i and LIF. Scale bar, 200 μm. (B) Quantitative real-time PCR to examine the mRNA level of <i>Yap</i>, <i>Pou5f1</i> and <i>Nanog</i> in wild type ES cells and <i>Mst-/</i>- ES cells during ES cell differentiation medium for 12 hours and 24 hours. <i>Actin</i> was analyzed as an internal control. The data are shown as the mean ± S.D (n=3). Statistically significant differences are indicated (*, P<0.05; **, P<0.01; ***, P<0.001). (C) Immunoblotting and densitometric analysis of Yap, YapS127, Oct4 and Nanog in wild type ES cells and <i>Mst-/</i>- ES cells in ES cell differentiation medium for 12 hours and 24 hours. Gapdh1 was analyzed as an internal loading control. The data are shown as the mean ± S.D (n=2). Statistically significant differences are indicated (*, P<0.05; **, P<0.01; ***, P<0.001).