Notch signaling was expressed and activated in BV-2 cells following hypoxia.

<p>(<b>A</b>) RT-PCR analysis showing the mRNA expression of Notch-1, Delta-1 and Hes-1 mRNA in BV-2 cells exposed to hypoxia was significantly increased compared with the control. (<b>B</b>) Western blotting of Notch-1, NICD, RBP-Jκ and Hes-1 protein expression in BV-2 cells exposed to hypoxia for 4, 6, 8 and 12 h and control (c). The left panel shows specific bands of Notch-1 (120 kDa), NICD (80 kDa), RBP-Jκ (56 kDa), Hes-1 (37 kDa) and β-actin (43 kDa). The right panel is bar graphs showing significant changes in the optical density following hypoxic exposure. Note significant increase in Notch-1, NICD, RBP-Jκ and Hes-1 expression after hypoxic treatment of varying durations in BV-2 cells. Significant differences between control and hypoxic BV-2 cells are expressed as *<i>p</i><0.05 and ** <i>p</i><0.01. The values represent the mean ±SD in triplicate.