The effects of SPRY4-IT1 on HTR-8/SVneo cell proliferation and migration.

<p>The expression of SPRY4-IT1 in the HTR-8/SVneo cells transfected with siRNA targeting SPRY4-IT1 (A left) and plasmid overexpressing SPRY4-IT1 (A right), detected by qRT- PCR. B: The proliferation was increased in si-SPRY4-IT1 group as compared to that of the negative control (left) while it was decreased in the group with SPRY4-IT1 overexpression (right) as compared to the cells transfected with GFP as identified by MTT assays. C&D: The migration capacity of the cells transfected with si-SPRY4-IT1 was significantly higher than that of the negative control (C) and lower in the cells overexpressing SPRY4-IT1 (D), as determined by Transwell assays (Values are mean±SEM;*: P<0.05; **: P<0.01).