p38 signalling mediates UV induced localisation of NR4A proteins to sites of DNA damage repair.

<p>(<b>A</b>) Fluorescent images of EYFP-NR4A protein localisation in nuclei following UVR treatment in the presence of inhibitors of p38 (SB203580; SB), PKA (H89), PKC (GF109203X; GF) or MEK1/2 (PD98059; PD) or DMSO (Vehicle). Representative images from 4 independent experiments are shown. (<b>B</b>) Nuclear images were scored as diffuse or foci positive from vehicle and p38 inhibitor treatments described above in mock and UVR treated cells. Bar graph shows the mean percentage of foci positive cells (+/− SEM). (<b>C</b>) Schematic representations of NR4A2 protein showing position of potential of Alanine substitutions at p38 phosphorylation sites in the N-terminal domain at amino acids S126 and S181. Clustal alignment below shows sequences flanking the serine residues (arrow head) and phospho sites (underlined). (<b>D</b>) Scoring of foci containing nuclei in cells expression WT and S126A, S181A and S126A/S181A mutant proteins as indicated. Bar graph represents the mean +/− SEM of results from 4 independent experiments. Statistical analysis in panels (B) and D was performed using ANOVA with a Tukey’s post-test (***, p<0.0001; **, p<0.001; *, p<0.01; ns, not significant).