CD8⁺ T-cell expression of ADAM17 is required for TNF-α processing following antigen recognition <i>in vivo</i>.

<p>SPC-HA transgenic mice received 10⁷ WT or ADAM17^−/− HA-specific CD8⁺ T cells via tail vein injection. ELISA was used to assess (A) TNF-α and (B) IFN-γ expression in cell-free BAL fluid. Alternatively, HA-specific CD8⁺ T cells were labeled with CFSE prior to transfer. Twenty-four hours after transfer, cells were recovered from (C) BAL and (D) lung homogenates and assessed by flow cytometric analysis to determine the total number of transferred HA-specific CD8⁺ T cells present. Data represent mean ± standard deviation. Data are representative of two or more independent experiments with 4 mice per group. **<i>P</i><0.01.