<i>Bursaphelenchus xylophilus</i> dead (stripes) and alive (grey) after the different <i>in</i><i>vitro</i> treatments with <i>Serratia</i> strain A88copa13 (growth supernatant) and with proteases (FPLC fractions), after 48 h incubation.

<p>The fractions with proteolytic activity contained 0.1M NaCl. (A) H₂O + Bx-Portugal11AS; (B) FPLC fraction containing the proteins that did not bound to the column + Bx-Portugal11AS; (C) Tris-HCl + (1M) NaCl + Bx-Portugal11AS; (D) Tris-HCl + (0.7M) NaCl + Bx-Portugal11AS; (E) Tris-HCl + (0.5M) NaCl + Bx-Portugal11AS; (F) Tris-HCl + (0.3M) NaCl + Bx-Portugal11AS; (G) Tris-HCl + (0.1M) NaCl + Bx-Portugal11AS; (H) FPLC fraction 19 + Bx-Portugal11AS; (I) <i>Serratia</i> strain A88copa13 (growth supernatant); (J) FPLC fraction 19 (75%) diluted with H₂O; (K) FPLC fraction 19 (50%) diluted with H₂O; (L) H₂O + Bt-Italy; (M) FPLC fraction 19 + Bt-Italy; (N) H₂O + Bc-Japan; (O) FPLC fraction 19 + Bc-Japan; (P) H₂O + Bm-Portugal2; (Q) FPLC fraction 19 + Bm-Portugal2. Results from triplicate assays. Different symbols (*, †, &) above the bars indicate significantly different percentages of mortality (P < 0.05) between treatments.