Proteins in the supernatants of nematicidal bacteria.
- Gabriel Paiva (481030)
- Diogo Neves Proença (358913)
- Romeu Francisco (358916)
- Paula Verissimo (481031)
- Susana S. Santos (481032)
- Luís Fonseca (5651308)
- Isabel M. O. Abrantes (358921)
- Paula V. Morais (289414)
Publication date
November 2013
DOIAbstract
<p>(A) SDS-PAGE of extracellular proteins from <i>Serratia</i> strains A88copa13 (2 and 3) and A88copa5 (4–6), molecular weight marker (1). Arrows indicate the band of 70 kDa (A-serine protease) and the band of 50 kDa (B-metalloproteinase serralysin) removed for sequencing by MALDI-TOF/TOF (MS / MS). (B) Zymogram using 12.5% polyacrilamide gel containing 0.2% copolymerized gelatin of extracellular proteins of <i>Serratia</i> strains A88copa13 (1 and 2) and A88copa5 (3 and 4) showing differences in protease activity.</p
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