Purification and characterization of the proteases in the growth supernatant of strain <i>Serratia</i> A88copa13.

<p>(A) Anionic exchange chromatography on Hitrap Q column (1ml). Fractions 9 and 19 were eluted by using a linear gradient 0-50% of 50 mM Tris HCl (pH 8.0) with 1 M NaCl for 20 minutes at 1ml/ min flow rate. FPLC protein fraction 9 rich in metalloproteinase (1), FPLC protein fraction 19 rich in metalloproteinase and serine protease (2), A88copa13 supernatant (3), and molecular weight (MW) evaluated by SDS-PAGE silver stained (B) and by zymography using 12.5% polyacrilamide gel containing 0.2% copolymerized gelatin (C).