Cell cycle ChIP assays to monitor association of DNA polymerases with telomeres in <i>trt1Δ</i> and <i>trt1-D743A</i> cells.

<p>(<b>A, B</b>) Peak normalized (A) or telomere length corrected (B) ChIP data for DNA polymerases. Raw ChIP data and % septated cells to monitor cell cycle progression are shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003936#pgen.1003936.s020" target="_blank">Figure S20A</a>–D. For peak normalized Pol1 (α), Student's t-test found p = 0.06 at 100 min (94% confidence level) and p = 0.03 at 120 min (97% confidence level) for wt vs. <i>trt1Δ</i> cells, and p = 0.02 at 100 min (98% confidence level) and p = 0.05 at 120 min (95% confidence level) for wt vs. <i>trt1-D743A</i> cells. For peak normalized Pol2 (ε), Student's t-test found p = 0.07 at 100 min (93% confidence level) for wt vs. <i>trt1Δ</i> cells, and p = 0.21 at 100 min (79% confidence level) for wt vs. <i>trt1-D743A</i> cells. For telomere length corrected Pol1 (α), statistically significant differences were found at 120 (p = 4.1×10⁻⁴), 140 (p = 5.3×10⁻³) and 160 min (4.5×10⁻²) for <i>trt1Δ</i> vs. <i>trt1-D743A</i> cells. Anti-FLAG western blot analysis indicated comparable expression levels in different genetic backgrounds (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003936#pgen.1003936.s020" target="_blank">Figure S20F</a>). (<b>C</b>) Comparison of peak normalized ChIP data for Trt1^TERT and DNA polymerases in <i>wt</i>, <i>trt1Δ</i>, and <i>trt1-D743A</i> cells. (Data for wt is identical to <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003936#pgen-1003936-g002" target="_blank">Figure 2D</a>, but shown again as a reference.) Statistically significant differences (p<0.04) in telomere binding between Pol1 (α) and Pol2 (ε) were found at 100 and 140–180 min for <i>trt1Δ</i> cells, and at 100, 200 and 220 min for <i>trt1-D743A</i> cells. Error bars correspond to SEM.