IL-10 mRNA levels on day 2 and 5 post infection after depletion of selective cell populations.

<p>(A–D) IL-10 mRNA levels in the spleen were determined on day 2 post LCMV Clone 13 infection by qPCR. (A) C57BL/6 mice were αLy6G, αCD4, αCD20 or αCD8 depleted or left untreated. (B) C57BL/6 mice were αNK1.1 or αCD25 depleted or left untreated. NK depletion: One representative of three experiments depicted. (C) <i>CD11c-DTR</i>⁺ and <i>CD11c-DTR</i>⁻ mice were treated with diphtheria toxin. One representative of two experiments is shown. (D) C57BL/6 mice were treated with clodronate containing liposomes or empty control liposomes. One representative of three experiments is shown. (E–H) IL-10 mRNA levels in the spleen were determined on day 5 post infection with LCMV Clone 13 by qPCR. C57BL/6 mice were (E) αCD8 or (F) αCD4 depleted or left untreated. (G) <i>Il-10</i>^fl/flx<i>CD11c-Cre</i>⁺ and <i>Il-10</i>^fl/flx<i>CD11c-Cre</i>⁻ mice. (H) C57BL/6 mice were treated with clodronate containing liposomes or empty control liposomes. The relative IL-10 expression was normalized to β-actin. The fold induction relative to the levels in naïve C57BL/6 is shown. A naïve <i>Il-10</i>^−/− mouse served as negative control. Each symbol represents one mouse. One representative of two is shown for each experiment.