Schematic illustration of fluorescence system.

<p>(a) Schematics of the tracking setup. Light emitted from the excitation source (a light-emitting diode array – LED array) causes the quantum dots to fluoresce. The emitted fluorescence is filtered and focused on a camera sensor. (b) Quantum dots are excited in the emission range of a blue LED array (blue line) filtered by a bandpass filter to out light of unwanted wavelengths (white area, left side of the panel). The blue area represents the light available for the excitation; the light absorbed by the quantum dots is represented by the purple area below the quantum dot absorbance spectrum (purple line). The emission spectrum of the quantum dot fluorescent at 585 nm and 655 nm (yellow and red lines, respectively) are filtered with a longpass filter (white area, right side of the panel) before being recorded by the camera sensor. All the curves are normalized versus the excitation light (peak at 465 nm). (c) Illustration of camera and aquarium set-up seen from above, showing the placement of, cameras, lenses, camera filters and aquarium.