Analysis of immune cells recruited to the spleen after infection with <i>Y. pseudotuberculosis</i> YPIII or YP147.

<p>About 2×10⁸ bacteria (YPIII, YP147) were used to infect BALB/c mice. Three days after infection, mice were sacrificed, the spleens were isolated, homogenized and the cell suspensions were used for flow cytometric analysis. Values on the y axis indicate the numbers of cells isolated from spleen infected with the wild-type strain YPIII or the <i>cnfY</i> mutant strain YP147. CD11b⁺/Ly6G⁻: macrophages/monocytes; CD11b⁺/Ly6G⁺: neutrophils; CD11c⁺: DCs; NKp46⁺: NK cells, CD3⁺: T cells, CD19⁺: B cells. The data show the median from at least two different experiments each done with groups of 4–6 mice. The asterisks indicates that there was a significant difference in the number of the indicated cells type in the whole organ in YP147-infected compared to YIPIII-infected mice based on a Mann-Whitney test with * (P<0.05), ** (P<0.01) and *** (P<0.001).