Effect of FCCP on mitochondrial membrane potential (Δ<i>Ψ</i>_m) and mPTP opening in isolated mouse ventricular myocytes.

<p><b>A</b>. TMRM fluorescence was monitored as an indicator of <i>Δψ</i>_<i>m</i>. FCCP (1 and 10 µM) was perfused as indicated by the horizontal bar. Two snapshots of TMRM fluorescence (control and ~2.5 min after the treatment with 10 µM FCCP) are shown. The fluorescence in the presence of 30 µM FCCP was set as 100% dissipation in each cell. <b>B</b>. Calcein fluorescence intensity was monitored as an indicator of mPTP opening. <b>B</b>-<b>a</b>. Two snapshots of calcein fluorescence at baseline (0 min) and 6 min after the treatment with FCCP (1 and 10 µM) and FCCP (1 µM) + CsA (1 µM). <b>B</b>-<b>b</b>. A representative recording of calcein fluorescence showing the rate of fluorescence decline in the presence of 1 and 10 µM FCCP. <b>B</b>-<b>c</b>. Summary data showing the calcein fluorescence decline in the presence of FCCP (100 nM, 1 and 10 µM) and CsA + FCCP as indicated. The fluorescence in the presence of 30 µM FCCP was set as 0% in each cell. *<i>p</i> < 0.05 vs. Control; ^#<i>p</i> < 0.05 vs. FCCP (1 µM).