GDF11 significantly attenuates Cor-1 cell proliferation in a dose-dependent manner.

<p>Cor-1 cells were grown in 96-well plates with cell number determined relative to control cultures using the MTS assay (see Methods). A representative experiment showing GDF11-dependent inhibition of Cor-1 cell proliferation over a 72 hr period is shown in (<b>A</b>). Each data point shows the mean relative optical density (+/−SEM) from 16 replicate cultures. (<b>B</b>) Incubation with either 25 ng/ml or 50 ng/ml of GDF11 significantly inhibits Cor-1 cell proliferation as monitored by MTS assay at the 48 hr time point. Data were normalised to control values for each of 5 independent experiment (mean ± SEM; ***<i>p</i><0.001, n = 5). (<b>C</b>) The inhibition of proliferation caused by GDF11 (50 ng/ml) measured at the 48 hr time point can be prevented in a dose-dependent manner by the GDF8 propeptide (PP). Shown here are results from a representative experiment, with each data point representing the mean +/− SEM relative to the control determined from 16 replicate cultures.