Thermal denaturation of the HMGB1 protein.

<p>A) The Trp fluorescence emission spectra of HMGB1 (black circles) and HMGB1ΔC (red circles) at each temperature were acquired and converted into CM and α according to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079572#eqn1" target="_blank">Equations 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079572#eqn2" target="_blank">2</a>, respectively. The curves were adjusted by sigmoidal fitting, and the T_m was obtained directly from the fitting. B) The CD signal at 222 nm for the HMGB1 and HMGB1ΔC spectra at each temperature was converted into the loss of secondary structure content. The buffer contained 10 mM Tris.HCl at pH 7.2, 50 mM NaCl, 0.5 mM DTT, 0.1 mM EDTA and 5% of glycerol.