HCF-1 regulates expression of Pdx-1.

<p>(A) Western blot analysis of Pdx-1 protein levels in HCF-1 knockdown cells. (B) Relative Pdx1 protein levels as determined by densitometry analysis. Pdx1 levels were normalized to β-actin levels. Results are pooled from 5 experiments and represent mean +/− SEM. **denotes a p-value = 0.03, ***denotes a p-value = 0.05 (Student’s t-test). (C) RT-qPCR analysis of <i>Pdx1</i>, <i>Ins1</i> and <i>Ins2</i> transcript levels in HCF-1 knockdown cells. The mean normalized RNA level for each gene in sicontrol treated cells was set to 1. The data shown are pooled from three independent experiments and are represented as mean +/− SEM. *denotes a p-value <0.05 relative to cells treated with sicontrol siRNA (Student’s t-test).