Analysis of blots and the schematic diagram of PKCα distribution and its effect on membrane.

<p>(A and B), total band intensities in three fractions were considered as 1, and the percentage of the light band (non-phosphorylated forms) and the heavy band (phosphorylated forms) were analyzed in three separate experiments. Schematic diagram, the PKCα was divided into two groups as “np-PKCα” (non-phosphorylated forms) and “p-PKCα” (phosphorylated forms). Per, represents peripheral membrane proteins; and IM, integral membrane proteins. The proportion and distribution of the two forms of PKCα were changed due to short/long term activation by PMA. The level of membrane-associated PKCα is inversely proportional to lateral membrane mobility.