Recovery of inflammatory cells from TME by flow cytometry.

<p>LLC-Luc cells were injected into left lung lobes of WT or cPLA₂-KO mice and tumor-bearing lungs were harvested 2.5 weeks later. Tissues from 4 mice were pooled, and inflammatory cells were recovered by flow cytometry. A. Sequential flow cytometry gating strategy used to recover inflammatory cells: Neu: neutrophils (CD11b+/Ly6G+), MacA macrophages (SigF+/CD11c+/Ly6G-) and MacB macrophages (F480+/CD11b+/Ly6G-/SigF-). B. Numbers of cells recovered by flow cytometry from uninjected or tumor-bearing left lung lobes of WT and cPLA₂-KO mice. Data show average from 3 separate experiments, with each sorting performed on a pool of 4 mice. Error bars show S.E.M.