Suppressor CD4+CD25+ T cells secrete effector cytokines in the presence of DC1 dendritic cells.

<p>(<i>5A</i>) 2.5×10⁵ sorted CD4+CD25+ (T_reg) or CD4+CD25− (T_eff) T cells were combined with 2.0×10⁵ immature or DC1 dendritic cells. Supernatants were harvested at 5 days and ELISA was used to measure the amount of IFN-γ present in the supernatant. Data shown are the average of at least four experiments. (<i>5B</i>) At day 5, some culture samples were permeabilized and intracellular IFN-γ was detected by flow cytometry. (<i>5C&D</i>) 2.5×10⁵ sorted CD4+CD25+ T cells were cocultured with immature (<i>5C</i>) or DC1 (<i>5D</i>) dendritic cells; intracellular expression of FoxP3 and IFN-γ was detected in permeabilized cells 5 days later.