Inhibition of T_reg function by DC1 dendritic cells is not due to apoptosis.

<p>1.25×10⁵ sorted, purified CD4+CD25+ T cells were co-cultured with 1×10⁵ immature dendritic cells (<i>2A</i>) or DC1 dendritic cells (<i>2B</i>). Expression of the apoptotic markers Annexin-V and 7-AAD 24 hours later is shown. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0074698#pone-0074698-g002" target="_blank">Figure 2C</a> summarizes the percent of cells expressing both markers (+/+), Annexin-V only (+/−), 7-AAD only (−/+), or neither marker (−/−). (iDC black, DC1 gray; <i>N = 4</i>).