Downstream target proteins such as c-Myc, Cyclin-D1 in the Wnt signal pathway.

<p>As c-Myc and Cyclin-D1 are mostly regarded as Wnt target genes, we further examined their expressions by western blot repeated for three times. Immunoblot analysis showing that Both c-Myc and cyclin-D1 increased at the Twist2/SKOV-3 group compared with the Vector/SKOV-3 group in the representative figure.