Heterozygous deletion of the SLD5 gene does not affect bone marrow cell reconstitution after their ablation by 5-FU.

<p>5-FU was injected into 8-week-old wild-type (+/+) and SLD5^+/− (+/−) mice and bone marrow cells were collected thereafter. (A) Kinetics of total bone marrow cell changes after 5-FU injection. Representative data from flow cytometric analysis (left) and quantitative evaluation of frequency in the leukocyte population (right) are shown. (B-D) Time course of the frequency of HSCs during bone marrow reconstitution after 5-FU injection. (B) Representative data of flow cytometric analysis (left) and quantitative evaluation of the frequency of KSL cells (right) gated as shown in the box in the left panels are shown. (C) Flow cytometric analysis of CD34 and c-Kit expression in KSL cells on day 7 after 5-FU injection (left). The frequency of CD34⁺ or CD34⁻ KSL cells was quantitatively evaluated (right two graphs). (D) Flow cytometric analysis of CD11b and c-Kit expression in Lineage marker⁻Sca-1⁺ cells after 5-FU injection (left). The frequencies of CD11b^lowKSL are quantitatively evaluated (right). Data are represented as mean ± SD (n = 3–7 mice in each group). Notably, no significant differences in the frequency between wild-type and SLD5^+/− mice were found at any time point.