p53 and UTX are recruited in an interdependent manner to the <i>ku80</i> promoter region.

<p>(<b>A</b>) <i>ku80</i> expression following IR exposure in Kc cells subjected to RNAi treatment, as indicated. (<b>B, C</b>) ChIP analysis of the physical occupancy of p53 and UTX at the <i>ku80</i> promoter region. Note that knockdown of <i>utx</i> eliminates the increase in p53 binding, and knockdown of <i>p53</i> reduces the binding of UTX to the <i>ku80</i> promoter. (<b>D</b>) ChIP assay for H3K27me3 at the <i>ku80</i> promoter in Kc cells treated with control or <i>utx</i> RNAi after IR. (<b>E</b>) Coimmunoprecipitation was performed using anti-p53 and anti-UTX antibodies and whole cell extracts of Kc cells. The immunoprecipitates were subjected to Western blot analysis with the indicated antibodies. (<b>F, G</b>) Western blot analysis to confirm the knockdown efficiency of <i>p53</i> RNAi. β-Tubulin (β-Tub) levels were used as a loading control.